Affinity chromatography of creatine phosphokinase on organomercurial-sepharose
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منابع مشابه
Affinity chromatography of viral DNA polymerases on pyran-sepharose.
Pyran covalently linked to cyanogen bromide-activated Sepharose has been shown to be an effective affinity matrix for several viral DNA polymerases. Differential salt elution of viral compared with cellular polymerases, as well as substrate elution, suggests the affinity nature for the matrix. Unlike some other affinity systems described, pyran-Sepharose is totally resistant to nuclease digesti...
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The specificity and sensitivity of serum creatine phosphokinase cardiac specific isoenzyme (MB) in the diagnosis of acute myocardial infarction (AM I) was evaluated. An ion-exchange chromatographic technique was used to isolate MB. Sera layered on mini-columns of DEAE-Sephadex were eluted with Tris-buffered sodium chloride. Quantification of isolated MB was performed by creatine phosphokinase (...
متن کاملSerum Levels of Creatine Phosphokinase.
Increasing interest in serum enzyme levels in various conditions has led to recognition of the value of the estimation of creatine phosphokinase activity in muscular disorders. Interpretation of the results obtained has been hampered by the lack of adequate information about the ;normal range'. Data for the normal levels of creatine phosphokinase in cord blood, children, and adults are presented.
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1975
ISSN: 0014-5793
DOI: 10.1016/0014-5793(75)80692-2